Micro-Volume Couette Flow Sample Orientation for Absorbance and Fluorescence Linear Dichroism

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Micro-volume couette flow sample orientation for absorbance and fluorescence linear dichroism.

Linear dichroism (LD) can be used to study the alignment of absorbing chromophores within long molecules. In particular, Couette flow LD has been used to good effect in probing ligand binding to DNA and to fibrous proteins. This technique has been previously limited by large sample requirements. Here we report the design and application of a new micro-volume Couette flow cell that significantly...

متن کامل

Protein fiber linear dichroism for structure determination and kinetics in a low-volume, low-wavelength couette flow cell.

High-resolution structure determination of soluble globular proteins relies heavily on x-ray crystallography techniques. Such an approach is often ineffective for investigations into the structure of fibrous proteins as these proteins generally do not crystallize. Thus investigations into fibrous protein structure have relied on less direct methods such as x-ray fiber diffraction and circular d...

متن کامل

Macro-Micro Simulation for Polymer Crystallization in Couette Flow

Polymer crystallization in manufacturing is a process where quiescent crystallization and flow-induced crystallization coexists, and heat/mass transfer on a macroscopic level interacts with crystal morphology evolution on a microscopic level. Previous numerical studies on polymer crystallization are mostly concentrated at a single scale; they only calculate macroscale parameters, e.g., temperat...

متن کامل

Linear Dichroism and Orientation of the Phycomyces Photopigment

The greater sensitivity of a cylindrical Phycomyces sporangiophore to blue light polarized transversely rather than longitudinally is a consequence of the dichroism and orientation of the receptor pigment. The abilities of wild type and several carotene mutants to distinguish between the two directions of polarization are the same. The E-vector angle for maximum response relative to the transve...

متن کامل

A linear absorbance converter for continuous-flow colorimeters.

Chronic glomerulonephritis Travenol coil 21h 5 n.d.c 14 14 0.833 Systemic lupus erythematosus Dow 27h 1 3 3 11 2.249 Chronic glomerulonephritis Dow 30h 15 2 8 n.d. 1.548 Chronic glornerulonephritis Dow or Kiil 26h 0 0 12 12 1.527 Rena’ carcinoma Dow or Kiil 20h 6 0 0 n.d. 1.253 Systemic lupus erythematosus Dow 18h 6 n.d. 3 55 0.554 Hypertensive nephropathy Dow 24h 1 5 12 23 1.621 Malignant hype...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biophysical Journal

سال: 2004

ISSN: 0006-3495

DOI: 10.1529/biophysj.103.035022